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» NZYMidiprep Kit

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  • Cat No.
  • PRO-MB05002-50

Catalogue No.:

Product Name:
NZYMidiprep Kit

Pack Size:
50 columns

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  • Product Specification Sheet




Description: NZYMidiprep kits are designed for the rapid, medium-scale preparation of highly pure plasmid DNA (typically 250 μg) from recombinant Escherichia coli strains.  Plasmid DNA binds selectively to NZYTech columns charged with a silica-based anion-exchange resin.  All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column.  In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer.  The purified nucleic acid products are suitable for use in the most demanding molecular biology applications, including transfection, in vitro transcription, automated fluorescent or manual sequencing, cloning, PCR and hybridization (Figure 1).  To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 200 ml of E. coli cultures and double the volumes of Buffers M1, M2 and M3.

Storage: All kit components can be stored at room temperature (20-25 °C) and are stable for up to one year.  For longer storage, keep all contents at 4 °C.