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» Pullulanase

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Under Development

Catalogue No.:
PRO-E0420

LOT:
2009-0420

Source Organism:
Cellvibrio japonicus NCIMB 10462

Enzyme Properties


EC No.:
3.2.1.41

Synonyms:
Limit dextrinase (erroneous); amylopectin 6-glucanohydrolase; bacterial debranching enzyme; debranching enzyme; α-dextrin endo-1,6-α-glucosidase; R-enzyme; pullulan α-1,6-glucanohydrolase; pullulan 6-α-glucanohydrolase; alpha-dextrin endo-1,6-alpha-glucosidase; pullulan alpha-1,6-glucanohydrolase; pullulan 6-alpha-glucanohydrolase

Nomenclature:
CAZy [GH13 subf13, glycoside hydrolase family 13 subfamily 13, member of clan GH-H] pul13B

Specific Activity:
-

Accession No.:
ACE82691

Molecular Weight:
166286.2 Da

Biological Function:
Catalyses the hydrolysis of (1→6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen

Comments:
Different to limit dextrinase (EC 3.2.1.142)

Primary Sequence


MLKYRNKLGLFALVLGLTALSGCGGGSGTGTDQVNFPKCQDPEVLLPGGQCGLPTEPFVPPACPDGQIRN
ALGVCIPSNFPTPVYKPGLNEAVIYVNVDGTESERKELLSKYNLHLWQACGGGWGNSVTDGKNTNYVIPT
TWPNGPFASSSGEPGAEKPHDPYYGAYFIIPISADGTCGNFIVKRPDLEPVLQTNDLRLTISRAGGQFDR
MVWVVINKDDMRNSIVSQVPACLRPDCSLERPITTITGIDAHWIDRDTIVWNKTIDPDYDVVLYQSAEAG
MSANTEGDIVGGQALATLSGARPMTEEEISRFPHLASYSAYDIPSTTGLDEIKTALKGELILYGRYDSVE
TETDPVTQEEQEVTVVRGYATRLQTAGALDDIYTSSDNDADEATLGVTYTAGGVGVSVWAPTAQNVELRV
FSGQPLRLAENIPMQLDTVTGIWHYQGTLAQLDRKFYRFRVTAFNAVDQRIRRLEVTDPYSISLATDGRY
SQFVNLNDADLKPSGWDEHTVPVAGAPENFVIYEAHVRDFSAQDESTPKAYRGKYMAFTVADSAPVNHLK
SLVESGLTHIHLLPTNDGGTVPEGAGSQVNLDSYIFELCQRVSNPADLSVCDGSIPNSKKVSEVIASFDT
KTSAARDLINAIKDIDGFNWGYDPVHYNAPEGSYATDSNGFVRVKEKRAMIMALHNIGLRTVFDVVYPHT
LESGNTSPNSTFDKIVPGYYFRTNVSTGLAENGTGAGSDTATEHRMMAKFVKDSLVHWTQNYKVDGFRFD
QSGYMPKAVLTESYDAVRAIDPDNYFYAEAWNPASPAPERIGLENLATQMSLAGTGIGTFNDRMRNPLRE
FQLFKGGSVDAIRAGLAGNLADFKLKAKNGAIISASTVGAYNLDPQEAINYVEKHDNETLWDWMHKPDAI
DAGTSIDNRVRMQNITLSIPVLSQGIPFIHMGSDLLRSKSMTADSYNAGDWFNYVDFTKQTNNWAVGLPP
RKEGMPSDAQILSAFNDVNSKPSPEHIEFAGEIFKEMLAIAKNSPLFSLQTAQEVFDRVGFQDGGKSQKA
GVIVMSIDDGAGVVSGTEDVQRADLDPALDAMVVVFNGTTTAQTLTVPTATGFELHDIQKNSVDEIVRNA
SFAEAVNDEIGGNFTVPAFTTAVFVKKQAGGQGVGLSAGATLDLPDQEPPPYASDVYVRGNVYDDDWSAV
DATRMAYHGRGIYSVVLDIDARVDAYKFKIAAADWNVPNLGANATVVLGAPLVLNQGGDSSDISINITES
GLYRFELDASSSLTAPTITVVKEQLNGGTVYLRGNIPEVGWDATTTLNQLSYTGKGIYSVAVDVAAAEDP
YSFKIASSDWSTFNFGNGSSIDLGEEAPVSQGGDNIGLTVSISATYRFELNMQDPAVPTVKVYADNVYSS
LPIYIRGNVSSDWGATDANQLAYSGSGLYTLKLNLAANNSYQFKVAAADWGALDFGSSNSAVLGTPVNLV
AGGSNIGLSIASEGEYVFTLDTANPEALTVTVDSAD