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» NZYLong DNA polymerase

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  • PRO-MB00302-500
    500U
    113.00

Catalogue No.:
PRO-MB00302

Product Name:
NZYLong DNA polymerase

Pack Size:
500 U


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Information:

Description: NZYLong DNA polymerase is a recombinant highly efficient thermostable DNA polymerase purified from Escherichia coli designed to amplify target DNA sequences ranging from 4 to more than 20 kb in size. NZYLong DNA polymerase displays 3'→5' exonuclease activity. Resulting blunt end polymerase chain reaction (PCR) products are suitable for cloning with NZYTech's NZYPCR cloning kit (MB01301 or MB01302).

Storage temperature: NZYLong DNA polymerase should be stored at -20 °C, in a constant temperature freezer. NZYLong DNA polymerase will remain stable up to 3 years if stored as specified.

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 1% (v/v) Triton X-100.

Enzyme concentration: 5 U/μl.

Reaction buffer (10x): 200 mM Tris-HCl, pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, 1 mg/ml of nuclease-free BSA. Vortex the Reaction Buffer solution thoroughly after thawing and prior to use. Repeated freeze-thaw cycles will affect the stability of the buffer (the buffer will remain stable at +4 °C for at least of one month).