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News - Biocatalysis and Advanced Enzyme Solutions

New Custom-made Enzyme Service

Posted Tue 21st Apr

Recombinant enzymeProzomix now offers a unique custom-made enzyme service to provide 100 mg of any* purified recombinant enzyme for £450!  A specific enzyme(s) can be identified by the customer, or a choice of targets (generated by bioinformatics searches, and checked for existing IP) suggested by Prozomix free-of-charge.  In all cases, the following conditions will apply:

1. Where it is not possible to provide 100 mg of the enzyme within 8 weeks, no charge will apply, and the customer will be informed immediately

2. Generally the enzyme will be available after 4-6 weeks, however, where certain technical issues are exprienced, up to 8 weeks (total elapsed time) may be required to complete the service

3. Most enzyme assays will be performed free-of-charge in order to confirm enzyme activity before shipping.  However, where an associated enzyme assay is either prohibitively expensive, or for technical or safety reasons can not be perfomed at Prozomix, a solution (e.g. a specific substrate is provided to Prozomix by the customer) will be negotiated before service work commences

4. This service is offered for enzymes of microbial origin only, or from other higher organisms where agreed in writing (please contact technical@prozomix.com)

*5. The offer of 100 mg for £450 is for enzymes of up to 30 kDa in size.  An additional charge of £50 applies for larger enzymes per extra 10 kDa of molecular mass (i.e. 100 mg of a 50 kDa enzyme would be £450 + (2 x £50) = £550)

6. The service is for 100 mg of purified recombinant enzyme ONLY, and in particular:

i. No clone/plasmid will be provided to the customer (all such materials will be the sole property of Prozomix at all stages of the service, and after the service has been provided)

ii. No enzyme production information will be provided

iii. After the service has been completed, the enzyme will be added to the general Prozomix product database, where other pack sizes will also become available in addition to 100 mg for £450 (specifically 1 mg for £10 and 10 mg for £75).  Note: all subsequent proceeds from the sale of the enzyme will belong entirely to Prozomix

 

(b) Bespoke Enzyme / Protein Service

Where the above "Custom-made Enzyme Service" does not meet your exact requirements, a bespoke and totally flexible purified enzyme / protein production service is available from Prozomix.  This service is also offered on a "no find, no fee" basis, and is expanded to include both native and recombinant enzymes / proteins. 

How your project might look:

Step 1. Customer sends email to technical@prozomix.com detailing the desired quantity and enzyme activity, and any other details:

e.g. Please provide a quote for the production of 100 mg of a secondary alcohol dehydrogenase (not from L. brevis)

Step 2. Within 24 hours, email sent back to customer with various options and prices:

e.g. We can offer you 100 mg of a secondary alcohol dehydrogenase that is either 91 %, 73 %, 49 % or 35 % identical to the enzyme from L. brevis.  In each case, the lead time will be 4-8 weeks, and the price would be:

a. £900 for 100 mg as either whole cells or unpurified cell-free extract (CFE), and details of the enzyme (microbial source, amino acid sequence, associated literature)

b. £950 for 100 mg of purified enzyme, and details of the enzyme (microbial source, amino acid sequence, associated literature)

c. £1950 for 100 mg of purified enzyme and the expression construct (with all necessary protein expression / purification protocols), and all details of the enzyme (microbial source, amino acid sequence, associated literature, etc)

Step 3. Customer chooses level of service:

e.g. Please quote for 100 mg of both the 73 % and 35 % identical targets as CFEs.

Step 4. Within 24 hours, email sent back to customer with detailed quote and prices:

e.g. The total price for the two enzymes will be £900 x 2 = £1800, less £100 discount = £1700.

Step 5. Customer accepts offer by email and receives weekly updates regarding progress of the order.  The two enzymes are shipped to the customer (at no further charge) within 6 weeks of the first email being sent.

For any further details regarding any of the enzyme services available from Prozomix, please contact technical@prozomix.com.

 

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